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About CCA!
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Voiceover
An aqueous solution of phenylhydrazine hydrochloride buffered with sodium acetate is added to three test tubes containing glucose, fructose, and sucrose. The solutions are heated in a boiling water bath. Reducing sugars do not form simple phenylhydrazone derivatives. Instead two molecules of phenylhydrazine condense with each molecule of sugar to produce a solid derivative called an osazone. A yellow solid rapidly forms with fructose and a short time later with glucose. Sucrose does not react for at least 30 minutes when it begins to hydrolyze.
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Citation:
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Shriner, R. L.; Fuson, R. C.; Curtin, D. Y.; Morrill, T. C.
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The Systematic Identification of Organic Compounds; 6th ed.; Wiley: New York, 1980; pp 261-262.
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Design, Text and Demonstrator:
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Gary Trammell
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University of Illinois at Springfield, Springfield, IL 62794
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Videographer/Editor:
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Steve Dykema
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University of Illinois at Springfield, Springfield, IL 62794
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Voice:
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Margaret Biddle
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University of Wisconsin - Madison, Madison, WI 53706
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Audio Production:
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Greg Minix
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University of Wisconsin - Madison, College of Engineering, Madison, WI 53706
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Jerrold J. Jacobsen
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University of Wisconsin - Madison, Madison, WI 53706
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