IMAGE. A beaker can be used as a developing chamber.
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Choose a developing chamber that can be sealed well. The chamber should be large enough to hold the paper that is to be developed.
Add the mobile phase to the chamber so that it is about 2 cm deep. Seal the chamber tightly and let the chamber stand until the atmosphere becomes saturated. For most general chemistry experiments, it should stand for approximately 15 minutes.
 Why should the developing chamber stand for 15 minutes? Answer
Allowing the chamber to stand permits its atmosphere to become saturated with the developing solvent. A saturated atmosphere allows for more effective development of the chromatograms. The larger the chamber, the longer it should stand.
What is wrong with the paper chromatography chamber shown below?
Answer
The chamber is uncovered, so it is not saturated with solvent vapor.
IMAGE. What is wrong with the paper chromatography chamber shown?
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